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Antibacterial and antiadhesive properties of carbon nanomaterials
Budil, Jakub
;
Lišková, Petra
(advisor) ;
Zikánová, Blanka
(referee)
Increasing interest in industrial and medical applications of carbon nanomaterial leads to the need to examine its interactions with living systems. Nanocrystalline diamond (NCD) films possess high mechanical and chemical stability which, together with its biocompatibility with human cells, enables applications in human body. Some of carbon nanoparticles possess strong antibacterial activity. In this work the effects of NCD with hydrogen, oxygen and fluorine termination deposited on glass and silicone on adhesion of gram-negative bacteria Escherichia coli K-12 in mineral medium is described and the impact of cultivation medium on effects of NCD films is compared. Prior the growth of the E. coli biofilm on NCD films, the method for quantification of biofilm using crystal violet staining and the method for biofilm cultivation in mineral medium were optimised. The properties of NCD film are independent on the base substrate. Hydrogen and fluorine terminated NCD films show antiadhesive properties only in mineral medium but not in complex medium. This is explained by formation of a conditioning film on the surface of the NCD film during cultivation in complex medium. On the other hand, O-NCD film supports bacterial adhesion in both cultivation media. Second part of this thesis is dedicated to carbon...
Detailed record
Interpretation of the C=O band of modified diamond nanoparticles by means of DFT theory
Jirásek, Vít
;
Kozak, Halyna
;
Remeš, Zdeněk
;
Kromka, Alexander
Diamond nanoparticles (DNPs) are an interesting class of nanomaterials, which has many unique properties. Their applications in medicine and molecular biology have been mentioned. The DNPs were reviewed in sense of their surface chemistry and a range of analytic techniques was pointed out. The experimental analysis can be enhanced by theoretical models based on the first principles. An approach to theoretical analysis of infrared spectra was introduced. As an example, a part of FTIR spectra of the oxidized DNPs was interpreted by means of the DFT theory. The calculation of the C=O stretch vibrational frequencies of carboxylic and anhydride groups on certain model clusters enabled to explain the shift and splitting of the measured spectral bands.
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Surface potentials of diamond nanoparticles of various size and termination
Arnault, J.-C.
;
Girard, H.
;
Petit, T.
;
Rezek, Bohuslav
Diamant nanoparticle potential similar as function of surface treatment. Diamant nanoparticle potential is similar on Au and Si substrate. KFM potential depends on particle/cluster size.
Detailed record
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